Accessing Olfactory Habituation in Drosophila melanogaster with a T-maze Paradigm
[Abstract] Habituation is the process whereby perceptual changes alter the value of environmental stimuli, enabling salience filtering. This behavioral response decrement is a form of non-associative learning, where the subject learns about the stimulus and does not involve sensory adaptation, sensory or motor fatigue. The range of behavioral responses in D. ...
Artificial Inhalation Protocol in Adult Mice
Authors: Thomas P. Eiting and
Matt Wachowiak,
date: 09/20/2018,
view: 1766,
Q&A: 0
[Abstract] Research in the area of in vivo olfactory physiology benefits from having direct access to the nasal airways through which odorants can be presented. Ordinarily, the passage of odorants through the airways is controlled by respiratory rhythm. This fact makes it difficult to control the timing and strength of an olfactory stimulus, since ...
Embryonic Intravitreous Injection in Mouse
Authors: Liyuan Cui,
Yupu Diao and
Jiayi Zhang,
date: 07/20/2018,
view: 1897,
Q&A: 0
[Abstract] Axons of retinal ganglion cells (RGCs) relay visual information from the retina to lateral geniculate nucleus (LGN) and superior colliculus (SC), which are two major image-forming visual nuclei. Wiring of these retinal projections completes before vision begins. However, there are few studies on retinal axons at embryonic stage due to technical ...
Dual-sided Voltage-sensitive Dye Imaging of Leech Ganglia
Authors: Yusuke Tomina and
Daniel A. Wagenaar,
date: 03/05/2018,
view: 3255,
Q&A: 0
[Abstract] In this protocol, we introduce an effective method for voltage-sensitive dye (VSD) loading and imaging of leech ganglia as used in Tomina and Wagenaar (2017). Dissection and dye loading procedures are the most critical steps toward successful whole-ganglion VSD imaging. The former entails the removal of the sheath that covers neurons in the ...