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Non-separate Mouse Sclerochoroid/RPE/Retina Staining and Whole Mount for the Integral Observation of Subretinal Layer

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Development and Standardization of Rapid and Efficient Seed Germination Protocol for Cannabis sativa

Authors: Aleksei Sorokin, Narendra Singh Yadav, Daniel Gaudet and Igor Kovalchuk, date: 01/05/2021, view: 433, Q&A: 0

[Abstract]

Cannabis seed germination is an important process for growers and researchers alike. Many biotechnological applications require a reliable sterile method for seed germination. This protocol outlines a seed germination procedure for Cannabis sativa using a hydrogen peroxide (H2O2) solution as liquid germination media. In this protocol, all three

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A Pulse–chase EdU Method for Detection of Cell Division Orientation in Arabidopsis and Juncus prismatocarpus Leaf Primordia

Authors: Xiaofeng Yin and Hirokazu Tsukaya, date: 01/05/2021, view: 254, Q&A: 0

[Abstract] In plants, the morphological diversity of leaves is largely determined by cell division, especially cell division orientation. Whereas cell division itself is easily monitored, the detection and quantification of cell division orientation are difficult. The few existing methods for detection and quantification of cell division orientation are ...

Gene Expression and Genome Editing Systems by Direct Delivery of Macromolecules Into Rice Egg Cells and Zygotes

Authors: Erika Toda and Takashi Okamoto, date: 07/20/2020, view: 878, Q&A: 0

[Abstract] Polyethylene glycol calcium (PEG-Ca²⁺)-mediated transfection allows rapid and efficient examination to analyze diverse cellular functions of genes of interest. In plant cells, macromolecules, such as DNA, RNA and protein, are delivered into protoplasts derived from somatic tissues or calli via PEG-Ca²⁺ transfection. To ...

Transient Expression Assay in Strawberry Fruits

Authors: Mengting Pi, Qi Gao and Chunying Kang, date: 06/05/2019, view: 3674, Q&A: 0

[Abstract] Strawberry, including the woodland strawberry Fragaria vesca (2x) and the cultivated strawberry (Fragaria × ananassa, 8x), has emerged as a model system for studying fruit development and ripening. Transient expression provides a quick assay for gene functions or gene interactions. In strawberry, ...

Visualization of Plant Cell Wall Epitopes Using Immunogold Labeling for Electron Microscopy

Author: Mateusz Majda, date: 04/05/2019, view: 3565, Q&A: 0

[Abstract] Plant cell walls consist of different polysaccharides and structural proteins, which form a rigid layer located outside of the plasma membrane. The wall is also a very dynamic cell composite, which is characterized by complex polysaccharide interactions and various modifications during cell development. The visualization of cell wall components in ...

Live Confocal Imaging of Brachypodium Spikelet Meristems

Author: Devin Lee O'Connor, date: 09/20/2018, view: 3426, Q&A: 0

[Abstract] Live confocal imaging of fluorescent reporters and stains in plant meristems provides valuable measurements of gene expression, protein dynamics, cell polarity, cell division, and growth. The spikelet meristem in the grass Brachypodium distachyon (Brachypodium) is well suited to live imaging because of the ease of dissection, small ...

Fabrication and Use of the Dual-Flow-RootChip for the Imaging of Arabidopsis Roots in Asymmetric Microenvironments

Authors: Claire E. Stanley, Jagriti Shrivastava, Rik Brugman, Elisa Heinzelmann, Viktoria Frajs, Andreas Bühler, Dirk van Swaay and Guido Grossmann, date: 09/20/2018, view: 5179, Q&A: 0

[Abstract] This protocol provides a detailed description of how to fabricate and use the dual-flow-RootChip (dfRootChip), a novel microfluidic platform for investigating root nutrition, root-microbe interactions and signaling and development in controlled asymmetric conditions. The dfRootChip was developed primarily to investigate how plants roots interact ...

Pollen Germination and Pollen Tube Growth of Arabidopsis thaliana: In vitro and Semi in vivo Methods

Authors: Hugh Dickinson, Josefina Rodriguez-Enriquez and Robert Grant-Downton , date: 08/20/2018, view: 9950, Q&A: 2

[Abstract] Studies of pollen germination and post-germination development are not only essential for understanding plant reproduction but also are an excellent model system for tip-based growth. Here we describe easy, reproducible methods for germination and growth of pollen from the model plant Arabidopsis thaliana in artificial conditions. Our ...

A Modified Approach for Axenic Cultivation of Spores of Fern Adiantum capillus-veneris L. with High Germination Rate

Authors: Yuan Ya-Ning, Yi Shu, Jin Wan-Ting and Fang Yu-Han, date: 07/05/2018, view: 2951, Q&A: 0

[Abstract] Spores are the primary way of spread and reproduction for ferns, a clade of seed-free vascular plants. However, no detailed protocol for ferns spore cultivation has been reported yet. Here we provide a modified approach for axenic cultivation of fern Adiantum capillus-veneris L., based on Cao’s and Li’s method (Cao, et al., 2010; ...

Micro-computed Tomography to Visualize Vascular Networks in Maize Stems

Authors: Akiteru Maeno and Katsutoshi Tsuda, date: 01/05/2018, view: 6215, Q&A: 0

[Abstract] Plant vascular systems in the stem connect roots with aerial organs to move solutes containing minerals, nutrients as well as signaling molecules, and therefore, they play pivotal roles in plant growth and development. However, stem vascular systems, especially in crop species, have been poorly described since they are deeply embedded in the ...

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