Measuring Protein Half-life in Arabidopsis thaliana
Authors: Hongqing Guo and
Yanhai Yin,
date: 08/05/2019,
view: 572,
Q&A: 0
[Abstract] Post-translational modifications play important roles in controlling protein function and can lead to altered protein stability. Protein stability can be determined after treatment with the protein synthesis inhibitor Cycloheximide. Cycloheximide is a translational inhibitor that inhibits protein synthesis via cytoplasmic ribosomes. Here we ...
Extraction and Purification of Laccases from Rice Stems
Authors: Chenna Swetha and
P. V. Shivaprasad,
date: 04/05/2019,
view: 1441,
Q&A: 0
[Abstract] Laccases are found in cell walls of plants in very low amounts. This protocol provides an efficient method to purify laccases from rice stems. The method involves three steps: 1) Isolation of total protein from rice stems using buffers with high salt concentration to extract protein from cell walls; 2) Purification of laccases using concanavalin-A ...
In planta Transcriptome Analysis of Pseudomonas syringae
Authors: Tatsuya Nobori and
Kenichi Tsuda,
date: 09/05/2018,
view: 3399,
Q&A: 0
[Abstract] Profiling bacterial transcriptome in planta is challenging due to the low abundance of bacterial RNA in infected plant tissues. Here, we describe a protocol to profile transcriptome of a foliar bacterial pathogen, Pseudomonas syringae pv. tomato DC3000, in the leaves of Arabidopsis thaliana at an early stage of ...