Neuroscience

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    Protocols in Current Issue
    Accessing Olfactory Habituation in Drosophila melanogaster with a T-maze Paradigm
    Authors:  Ourania Semelidou, Summer F. Acevedo and Efthimios M.C. Skoulakis, date: 06/05/2019, view: 140, Q&A: 0
    Habituation is the process whereby perceptual changes alter the value of environmental stimuli, enabling salience filtering. This behavioral response decrement is a form of non-associative learning, where the subject learns about the stimulus and ...
    Accessing Olfactory Habituation in Drosophila melanogaster with a T-maze Paradigm
    Authors:  Ourania Semelidou, Summer F. Acevedo and Efthimios M.C. Skoulakis, date: 06/05/2019, view: 140, Q&A: 0
    [Abstract] Habituation is the process whereby perceptual changes alter the value of environmental stimuli, enabling salience filtering. This behavioral response decrement is a form of non-associative learning, where the subject learns about the stimulus and does not involve sensory adaptation, sensory or motor fatigue. The range of behavioral responses in D. ...
    Protocol to Quantitatively Assess the Structural Integrity of Perineuronal Nets ex vivo
    Authors:  Bhanu P. Tewari and Harald Sontheimer, date: 05/20/2019, view: 310, Q&A: 0
    [Abstract] Perineuronal nets (PNNs) are extracellular matrix assemblies of highly negatively charged proteoglycans that wrap around fast-spiking parvalbumin (PV) expressing interneurons in the cerebral cortex. PNNs play important roles in neuronal plasticity and modulate biophysical properties of the enclosed interneurons. Various central nervous system ...
    Inducing Alcohol Dependence in Rats Using Chronic Intermittent Exposure to Alcohol Vapor
    Authors:  Elizabeth M. Avegno and Nicholas W. Gilpin, date: 05/05/2019, view: 289, Q&A: 0
    [Abstract] Alcohol use disorder (AUD) is a significant public health and economic burden and is often characterized by repeated bouts of alcohol intoxication and withdrawal. Virtually all organ systems are impacted by chronic alcohol exposure. These effects can be investigated using the rat as a model organism; however, rats typically will not ...
    Displaced Object Recognition Memory in Rats
    Authors:  Ali N. Mclagan and Jena B. Hales, date: 04/20/2019, view: 484, Q&A: 0
    [Abstract] The Displaced Object Recognition (DOR) task, sometimes called the Novel Object Location task, assesses spatial recognition memory without navigational demands, explicit instruction, or the need for multiple days of training. This memory task has two phases. First, the subject is familiarized to an open arena with two objects and is allowed to ...
    Preparation and Manipulation of Olfactory Epithelium Explant Cultures for Measurement of the Mechanical Tension of Individual Axons Using the Biomembrane Force Probe
    Authors:  Coralie Fouquet and Alain Trembleau, date: 04/20/2019, view: 289, Q&A: 0
    [Abstract] In this paper, we describe a protocol allowing measurement of the mechanical tension of individual axons grown ex vivo from neural tissue explants. This protocol was developed with primary cultures of olfactory epithelium explants from embryonic (E13.5) mice. It includes a detailed description of explant dissection and culture, as well as ...
    Time-lapse Whole-field Fluorescence Imaging of Microglia Processes Motility in Acute Mouse Hippocampal Slices and Analysis
    [Abstract] Microglia are the resident immune cells of the central nervous system (CNS). In the last year, the improvements in the transgenic mouse technologies and imaging techniques have shed light on microglia functions under physiological conditions. Microglia continuously scan the brain parenchyma with their highly motile processes, maintaining tissue ...
    Consummatory Successive Negative Contrast in Rats
    [Abstract] Using animal models in addiction and pain research is pivotal to unravel new pathways and mechanisms for the treatment of these disorders. Reward devaluation through a consummatory successive negative contrast (cSNC) task has shown the ability to reduce physical pain sensitivity (hypoalgesia) and increase oral ethanol consumption in rats. The ...
    Primary Embryonic Rat Cortical Neuronal Culture and Chronic Rotenone Treatment in Microfluidic Culture Devices
    Authors:  Victor S. Van Laar, Beth Arnold and Sarah B Berman, date: 03/20/2019, view: 675, Q&A: 0
    [Abstract] In the study of neurodegenerative diseases, it is imperative to study the cellular and molecular changes associated with pathogenesis in the relevant cell type, central nervous system neurons. The unique compartmentalized morphology and bioenergetic needs of primary neurons present complications for their study in culture. Recent microculture ...
    Social Defeat Stress (SDS) in Mice: Using Swiss Mice as Resident
    Authors:  Marco Oreste F. Pagliusi Jr. and Cesar R. Sartori, date: 03/20/2019, view: 757, Q&A: 0
    [Abstract] Due to the high prevalence and great economic impact of depression, studies with animal models have been increasingly used to identify neurobiological mechanisms associated with this disorder. However, many animal models use stressful conditions that are not consistent with what we observe in the modern human world. Examples are the chronic ...
    Acute Cerebellar Slice Preparation Using a Tissue Chopper
    Authors:  Yubin Wang and Michel Baudry, date: 03/05/2019, view: 560, Q&A: 0
    [Abstract] Acute cerebellar slices are widely used among neuroscientists to study the properties of excitatory and inhibitory synaptic transmission as well as intracellular signaling pathways involved in their regulation in cerebellum. The cerebellar cortex presents a well-organized circuitry, and several neuronal pathways can be stimulated and recorded ...



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