A Protocol to Map the Spatial Proteome Using HyperLOPIT in Saccharomyces cerevisiae
[Abstract] The correct subcellular localization of proteins is vital for cellular function and the study of this process at the systems level will therefore enrich our understanding of the roles of proteins within the cell. Multiple methods are available for the study of protein subcellular localization, including fluorescence microscopy, organelle ...
Quantitative ChIP-seq by Adding Spike-in from Another Species
Authors: Kongyan Niu,
Rui Liu and
Nan Liu,
date: 08/20/2018,
view: 3551,
Q&A: 0
[Abstract] Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is a routine procedure in the lab; however, epigenome-wide quantitative comparison among independent ChIP-seq experiments remains a challenge. Here, we contribute an experimental protocol combined with a computational workflow allowing quantitative and comparative assessment of ...
Bacterial Microcolonies in Gel Beads for High-throughput Screening
Author: Yolanda Schaerli,
date: 07/05/2018,
view: 3268,
Q&A: 0
[Abstract] High-throughput screening of a DNA library expressed in a bacterial population for identifying potentially rare members displaying a property of interest is a crucial step for success in many experiments such as directed evolution of proteins and synthetic circuits and deep mutational scanning to identify gain- or loss-of-function mutants.
...
Extraction and 16S rRNA Sequence Analysis of Microbiomes Associated with Rice Roots
[Abstract] Plant roots associate with a wide diversity of bacteria and archaea across the root-soil spectrum. The rhizosphere microbiota, the communities of microbes in the soil adjacent to the root, can contain up to 10 billion bacterial cells per gram of soil (Raynaud and Nunan, 2014) and can play important roles for the fitness of the host plant. Subsets ...