Aksiniya Asenova University of California, Davis
18 protocols

Alexandros Alexandratos National and Kapodistrian University of Athens
65 protocols

Alka Mehra Institute of Genomics and Integrative Biology
45 protocols

Amit Dey International Centre for Genetic Engineering and Biotechnology
18 protocols

Fanglian He
  • Publisher, Bio-protocol
Research focus
  • Host-bacterium interaction
  • 19 Author merit


Ph.D., University of Pennsylvania, USA


19 Protocols published
Alkaline Phosphatase
Author:  Fanglian He, date: 07/20/2012, view: 38907, Q&A: 4
Alkaline phosphatase removes 5' phosphate groups from vector so that prevents self-ligation of the vector and facilitates the ligation of other DNA fragments into the vector.
Oil Red O Staining of Fixed Worm
Author:  Fanglian He, date: 07/05/2012, view: 25561, Q&A: 1
Oil red O staining is used to assess major fat stores in C. elegans. This protocol is adapted from the Ashrafi Lab at the University of California-San Francisco.
63 Protocols reviewed
Quantitation of Cytochromes b559, b6, and f, and the Core Component of Photosystem I P700 in Cyanobacterial Cells
Authors:  Motohide Aoki, Mikio Tsuzuki and Norihiro Sato, date: 11/05/2016, view: 7372, Q&A: 0
Cytochrome (Cyt) b559, an important and essential core component of photosystem II in the photosynthetic electron transport system, is a heme-bridged heterodimer protein composed of an alpha subunit (PsbE) and a beta ...
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Quantification of Callose Deposition in Plant Leaves
Callose is an amorphous homopolymer, composed of β-1, 3-glucan, which is widespread in higher plants. Callose is involved in multiple aspects of plant growth and development. It is synthetized in plants at the cell plate during cytokinesis, in ...
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142 Protocols edited
Isolation of Highly Pure Primary Mouse Alveolar Epithelial Type II Cells by Flow Cytometric Cell Sorting
Authors:  Meenal Sinha and Clifford A. Lowell, date: 11/20/2016, view: 22832, Q&A: 1
In this protocol, we describe the method for isolating highly pure primary alveolar epithelial type II (ATII) cells from lungs of naïve mice. The method combines negative selection for a variety of lineage markers along with positive selection for ...
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Extraction and Quantification of Polyphosphate in the Budding Yeast Saccharomyces cerevisiae
Authors:  David Canadell, Samuel Bru, Josep Clotet and Joaquín Ariño, date: 07/20/2016, view: 10742, Q&A: 0
Inorganic polyphosphate (polyP) is a linear polymer present in both prokaryotic and eukaryotic organisms and made from three to hundreds of orthophosphate residues linked by phosphoanhydride bonds. The biological role of this molecule goes beyond ...
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