Education
PhD, AIIMS, India, 2015
Lab information
Nicola Pozzi Lab
https://biochem.slu.edu/people/pozzi/
Research focus
Structural and Functional role of B2-GPI in Anti-phospholipid Syndrome:
β2-glycoprotein I (β2GPI) is an abundant multidomain plasma protein that plays various roles in the clotting and complement cascades. Beta-2-Glycoprotein I (β2GPI) is a unique five domain protein comprising four similar complement control protein (CCP)-like domains (DI-DIV) and one different domain (DV) with a large lysine. It is a soluble blood protein with a circulating concentration of 0.2 mg/ml and a molecular weight of 50kDa with glycosylation. β2GPI has many proposed functions and roles within the body including the regulation of complement and hemostasis. It is also the main target of anti- phospholipid antibodies (aPL) in the acquired coagulopathy known as antiphospholipid syndrome (APS). Being an important target against APS, the structure and function of B2-GPI should be explored in detail to make it as therapeutic target to treat APS disease.
We are using the state-of-the-art biochemical and biophysical methods (Biochemical methods, SPR, X-ray crystallography, SAXS, Cryo-EM, smFRET) in our lab to study the dynamic behaviors, structure, and functions of proteins involved in antiphospholipid syndrome.
Publications
https://scholar.google.com/citations?user=Q2cK6GcAAAAJPUBLICATIONS
1. Suresh Kumar, Mathivanan Chinnaraj, William Planer, Xiaobing Zuo, Paolo Macor, Francesco Tedesco, and Nicola Pozzi1. An allosteric redox switch in domain V of β2-glycoprotein I controls membrane binding and anti-domain I autoantibody recognition. J Biol Chem. 2021 Aug; 297(2): 100890.
2. Kumar S, Akabayov SR, Kessler N. et al. The methyl 13C-edited/13C-filtered transferred NOE for studying protein interactions with short linear motifs. J Biomol NMR (2020). https://doi.org/10.1007/s10858-020-00340-y.
3. Prakash J, Yadav S, Saha G, Chiranjivi AK, Kumar S, Sasidharan S, Saudagar P, Dubey VK. Episomal expression of human glutathione reductase (HuGR) in Leishmania sheds light on evolutionary pressure for unique redox metabolism pathway: Impaired stress tolerance ability of Leishmania donovani. Int J Biol Macromol, 2019; 121:498-507.
4. Suresh Kumar, Vinay Kumar Sharma, Savita Yadav, Sharmistha Dey. Anti-proliferative and apoptotic effects of black turtle bean extracts on human breast cancer cell line through extrinsic and intrinsic pathway. BMC Chemistry, 2017, 11(1):56.
5. Suresh Kumar, Avinash Singh, Shavait Yamini, Sharmistha Dey, T. P. Singh. Crystal structure of Mg2+ containing hemopexin-fold protein from Kabuli Chana (Chickpea-white, CW-25) at 2.45Å resolution reveals its metal ion transport property. The Protein Journal, 2015; 34 (4): 284-290.
6. Suresh Kumar, Kumar Jitendra1, Kusum Singh, Vaishali Kapoor, Mou Sinha, Immaculata Xess, Satya N. Das, Sujata Sharma, Tej P. Singh, Sharmistha Dey. Biological properties and Characterization of ASL50 protein from aged Allium sativum bulbs. Appl Biochem Biotech 2015; 176 (7): 1914-1927.
7. Suresh Kumar, Vaishali Kapoor, Kamaldeep Gill, Kusum Singh, Immaculata Xess, Satya N. Das, Sharmistha Dey. Antifungal and Antiproliferative Protein from Cicer arietinum: A Bioactive Compound against Emerging Pathogens. Biomed Res Int 2014; 2014: 387203, DOI: 10.1155/2014/387203.
Suresh Kumar 3 of 6
8. Kusum Singh, Suresh Kumar, Shashank Shekhar, Benu Dhawan, Sharmistha Dey. Synthesis and biological evaluation of novel peptide BF2 as an antibacterial agent against clinical isolates of vancomycin-resistant enterococci. J Med Chem 2014; 57(21): 8880-8885.
9. Kamal Gill, Suresh Kumar, Immaculata Xess, Sharmistha Dey. Novel synthetic anti-fungal tripeptide effective against Candida krusei. Indian J Med Microbiol 2015; 33(1): 110-116.
10. Kamaldeep Gill, Lokesh Nigam, Ratnakar Singh, Suresh Kumar, Naidu Subbarao, Shyam Singh Chauhan, Sharmistha Dey. The rational design of specific peptide inhibitor against p38alpha MAPK at allosteric-site: a therapeutic modality for HNSCC. PLoS One 2014; 9 (7): e101525.
11. Kamal Gill, Abhay, Kumar Singh, Suresh Kumar, Biswajit Mishra, Vaishali Kapoor, Satya N Das, Rishi Kumar Somvanshi, Sharmistha Dey. Isolation and characterization of a potent protein from ginger rhizomes having multiple medicinal properties. Res J Med Plant 2012; 6 (2): 160-170.