Thank you for your question. I think A1.2 "Place samples in a thermocycler with a heated lid set to approximately 105°C. Heat the mixture to 95°C for 2 min, slowly cool to 22°C (-0.1°C/s), and incubate at 22°C for an additional 5 min" is the reason for your confusion.
Acutually, the 105°C lid is set for the high tempture reaction during the temperature gradient decline from 95°C to 22°C. 2022-05-03 06:31:15