Lab information
CSIR National Botanical Research Institute
Molecular Biology and Biotechnology division
Research focus
I did my Ph.D. work from CSIR-National Botanical Research Institute and was enrolled from Banaras Hindu University (2016). My research work focused on cotton tissue culture and transformation. During my doctoral work, I significantly improved the efficiency of cotton transformation in the cotton tissue culture lab at molecular biology and biotechnology division, CSIR-NBRI. My work yielded me first authorship in the research article “Expression of an insecticidal fern protein in cotton protects against whitefly, Nature Biotechnology. 34(10), 1046-1051”. I have been working in plant tissue culture and transformation for the last 16 years and have transformed many plants (cotton, tomato, tobacco, maize, rice, etc.). During my postdoctoral work, I also established the art of regeneration and transformation in maize at ICAR-Vivekananda Parvatiya Krishi Anusandhan Sansthan, Almora. In 2017, I again joined the CSIR-NBRI as a Project Scientist, after that I am working as Scientific Consultant in Cotton Mission Project. I have developed more than 300 transgenic cotton lines and done the molecular characterization of these GM lines. I have the expertise of contained field trails of selected GM cotton lines. I have knowledge in the field of biotechnology, plant tissue culture, microbiology, molecular biology, and contained field trails.
Publications
1. Anoop Kumar Shukla, S K Upadhyay, M Mishra, S Saurabh, R Singh, H Singh, N Thakur, P Rai, P Pandey, AL Hans, S Srivastava, V Rajapure, SK Yadav, MK Singh, J Singh, K Chandrashekar, PC Verma, AP Singh, KN Nair, S Bhadauria, M Wahajuddin, S Singh, S Sharma, Omkar, RS Upadhyay, SA Ranade, R Tuli, PK Singh, 2016, Expression of an insecticidal fern protein in cotton protects against whitefly, Nature Biotechnology. 34(10), 1046-1051.
2. M Kumar, H Singh, Anoop Kumar Shukla, PC Verma, and PK Singh 2013. Induction and establishment of somatic embryogenesis in elite Indian cotton cultivar (Gossypium hirsutum L. cv Khandwa-2). Plant signaling & Behavior. 8:10, e26762
3. M Kumar, Anoop Kumar Shukla, H Singh, PC Verma, PK Singh, 2013. A genotype-independent Agrobacterium-mediated transformation of germinated embryo of cotton (Gossypium hirsutum L.) International Journal of Bio-Technology and Research. 03; 3(1):81-90.
4. PC Verma, I Trivedi, H Singh, Anoop Kumar Shukla, M Kumar, SK Upadhyay, P Pandey, AL Hans, PK Singh., 2009. Efficient production of gossypol from hairy root cultures of cotton (Gossypium hirsutum L.). Current Pharmaceutical Biotechnology. 10 (7): 691-700.
5. M Kumar, Anoop Kumar Shukla, H Singh, R Tuli, 2009. Development of insect resistant transgenic cotton lines expressing cry1EC gene from an insect bite and wound inducible promoter. Journal of Biotechnology. 140(3-4):143-8.