Harrie van Erp Rothamsted Research
12 protocols

Marisa Rosa Bio-protocol.org
71 protocols

Matilda Chan University of California
1 protocol

Matthew Escobar California State University San Marcos
1 protocol

Reviewer
Lifeng Liu
  • Post-Doc, DOE Joint Genome Institute
Research focus
  • Molecular biology
  • epigenetics, gene regulation
4 Protocols reviewed
PCR-mediated One-day Synthesis of Guide RNA for the CRISPR/Cas9 System
Authors:  Naim Hassan, Farhana Easmin, Keisuke Ekino and Satoshi Harashima, date: 07/05/2021, view: 1085, Q&A: 0

Nowadays, CRISPR (clustered regularly interspaced short palindromic repeats) and the CRISPR-associated protein (Cas9) system play a major role in genome editing. To target the desired sequence of the genome successfully, guide RNA (gRNA) is

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Identification of Intrinsic RNA Binding Specificity of Purified Proteins by in vitro RNA Immunoprecipitation (vitRIP)
Authors:  Marisa Müller, Tamas Schauer and Peter B. Becker, date: 03/05/2021, view: 1332, Q&A: 0

RNA-protein interactions are often mediated by dedicated canonical RNA binding domains. However, interactions through non-canonical domains with unknown specificity are increasingly observed, raising the question how RNA targets are recognized.

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