Kyosuke Nagata Department of Infection Biology, Faculty of Medicine, University of Tsukuba, Japan
1 protocol
Alka Mehra Institute of Genomics and Integrative Biology
45 protocols

Arsalan Daudi University of California
157 protocols

Nathan James MRC Laboratory of Molecular Biology
3 protocols

Sonali Chaturvedi Gladstone Institute of Virology and Immunology
3 protocols

Kenji Sugiyama
  • Biotech Engineer, University of Tsukuba
Research focus
  • Influenza virus
  • 1 Author merit

Lab information

Nagata Lab

1 Protocol published
Purification and Identification of Novel Host-derived Factors for Influenza Virus Replication from Human Nuclear Extracts
Authors:  Kenji Sugiyama and Kyosuke Nagata, date: 09/20/2016, view: 9178, Q&A: 0
Recently, we identified two host cell-derived proteins as novel stimulatory factors of influenza virus RNA replication process, termed “Influenza virus REplication Factor-2 (IREF-2)”, from human nuclear extracts (NEs) by employing biochemical ...
3 Protocols reviewed
Colorimetric RT-LAMP and LAMP-sequencing for Detecting SARS-CoV-2 RNA in Clinical Samples

During pandemics, such as the one caused by SARS-CoV-2 coronavirus, simple methods to rapidly test large numbers of people are needed. As a faster and less resource-demanding alternative to detect viral RNA by conventional qPCR, we used reverse

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Ribosome Purification from an α-proteobacterium and rRNA Analysis by Northern Blot
Authors:  Liang Yin and Caroline S. Harwood, date: 12/05/2020, view: 1717, Q&A: 0
Ribosomes are an integral part of cellular life. They are complex molecular machines consisting of multiple ribosomal proteins and RNAs. To study different aspects of ribosome composition, many methods have been developed over the decades. Here, we ...
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