Akira Karasawa New York Structural Biology Center
10 protocols

Alba Blesa Universidad Francisco de Vitoria
42 protocols

Alexandros Kokotos Weill Cornell Medicine
18 protocols

Alka Mehra Institute of Genomics and Integrative Biology
45 protocols

Editor
Ralph Thomas Boettcher
  • Project group leader, Max Planck Institute for Biochemistry
Research focus
  • Cell biology
  • 1 Author merit
1 Protocol published
β1 Integrin Cell-surface Immunoprecipitation (Selective Immunoprecipitation)
Author:  Ralph T. Böttcher, date: 11/05/2013, view: 10807, Q&A: 0
Immunoprecipitation (IP) is a widely used method to isolate a specific protein from a mixed protein sample using an antibody that exclusively binds to that particular protein. This technique allows studying protein-protein and protein-nucleic acid ...
6 Protocols reviewed
Cell-free Synthesis of Correctly Folded Proteins with Multiple Disulphide Bonds: Production of Fungal Hydrophobins
Authors:  Rezwan Siddiquee and Ann H Kwan, date: 05/20/2021, view: 3637, Q&A: 0

Cell-free synthesis is a powerful technique that uses the transcriptional and translational machinery extracted from cells to create proteins without the constraints of living cells. Here, we report a cell-free protein production protocol using

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49 Protocols edited
A Fast and Reliable Method to Generate Pure, Single Cell-derived Clones of Mammalian Cells
Authors:  Zhe Han, Bindhu K. Madhavan, Serap Kaymak, Peter Nawroth and Varun Kumar, date: 08/20/2022, view: 1101, Q&A: 0

Stable cell cloning is an essential aspect of biological research. All advanced genome editing tools rely heavily on stable, pure, single cell-derived clones of genetically engineered cells. For years, researchers have depended on single-cell

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Immunoisolation of Endosomal Recycling Vesicles from Saccharomyces cerevisiae
Authors:  Sho W. Suzuki and Scott D. Emr, date: 05/05/2022, view: 1064, Q&A: 0

Endosomal recycling is essential for the appropriate function of the endosome. During this process, endosomal coat complexes (i.e., retromer, and Mvp1) are recruited to the endosome, and deform its membrane to form recycling vesicles. To further

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