Benita Westerlund-Wikström Department of Biosciences, University of Helsinki, Finland
1 protocol
Agnès Groisillier Sorbonne Universités, University Pierre and Marie Curie
3 protocols

Aksiniya Asenova University of California, Davis
18 protocols

Alba Blesa Universidad Francisco de Vitoria
39 protocols

Alexander Westbye NIOZ Royal Netherlands Institute for Sea Research
6 protocols

Reviewer
Timo A Lehti
  • Post-Doc, University of Helsinki
Research focus
  • 1 Author merit

Education

Ph.D., University of Helsinki

Publications

Lehti TA, Pajunen MI, Jokilammi A, Korja M, Lilie H, Vettenranta K & Finne J (2021) Design of a Cytotoxic Neuroblastoma-Targeting Agent Using an Enzyme Acting on Polysialic Acid Fused to a Toxin. Molecular Cancer Therapeutics 20:1996-2007.

Gucciardo E, Lehti TA, Korhonen A, Salvén P, Lehti K, Jeltsch M & Loukovaara S (2020) Lymphatics and the Eye. Duodecim 136:1777-1788.

Lehti TA, Pajunen MI, Skog MS & Finne J (2017) Internalization of a polysialic acid-binding Escherichia coli bacteriophage into eukaryotic neuroblastoma cells. Nature Communications 8:1915.

Uotila LM, Guenther C, Savinko T, Lehti TA & Fagerholm SC (2017) Filamin A regulates neutrophil adhesion, production of reactive oxygen species and neutrophil extracellular trap release. The Journal of Immunology 199: 3644-3653.

Skog MS, Nystedt J, Korhonen M, Anderson H, Lehti TA, Pajunen MI & Finne J (2016) Expression of neural cell adhesion molecule and polysialic acid in human bone marrow-derived mesenchymal stromal cells. Stem Cell Research & Therapy 7: 113.

Lehti TA, Bauchart P, Kukkonen M, Dobrindt U, Korhonen T, & Westerlund-Wikström B (2013) Phylogenetic Group –Associated Differences in Regulation of the Common Colonization Factor Mat Fimbria in Escherichia coli. Molecular Microbiology 87(6): 1200-1222.

Lehti TA, Bauchart P, Dobrindt U, Korhonen T, & Westerlund-Wikström B (2012) The fimbriae activator MatA switches off motility in Escherichia coli by repression of the flagellar master operon flhDC. Microbiology 158(6): 1444-1455.

Lehti TA, Heikkinen J, Korhonen T, & Westerlund-Wikström B (2012) The response regulator RcsB activates expression of Mat fimbriae in meningitic Escherichia coli. Journal of Bacteriology 194(13): 3475-3485.

Edelman S, Lehti TA, Kainulainen V, Antikainen J, Kylväjä S, Baumann M, Westerlund-Wikström B & Korhonen T (2012) Identification of a high-molecular-weight Lactobacillus Epithelium Adhesin (LEA) of Lactobacillus crispatus ST1 that binds to stratified squamous epithelium. Microbiology 158(Pt 7): 1713-1722.

Lehti TA, Bauchart P, Heikkinen J, Hacker J, Korhonen T, Dobrindt U & Westerlund-Wikström B (2010) Mat fimbriae promote biofilm formation by meningitis-associated Escherichia coli. Microbiology 156(8): 2408-2417.
1 Protocol published
Colony Immunoblotting Assay for Detection of Bacterial Cell-surface or Extracellular Proteins
Authors:  Timo A. Lehti and Benita Westerlund-Wikström, date: 09/05/2013, view: 26997, Q&A: 1
This simple protocol describes how to detect antigens from agar-grown bacterial colonies transferred to nitrocellulose using specific antibodies. The protocol is well suitable for detection of bacterial proteins exposed on the cell surface or ...
20 Protocols reviewed
Heterologous Expression and Purification of SARS-CoV2 Nucleocapsid Protein
Authors:  Ankur Garg, Lihong Liu, David D. Ho and Leemor Joshua-Tor, date: 08/05/2020, view: 3833, Q&A: 0
This protocol describes a step by step method for heterologous expression of SARS-CoV2 Nucleocapsid (N) protein in Escherichia coli. Moreover, this protocol includes steps to purify the N protein to high purity and homogeneity. Thus, ...
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Listeria innocua Biofilm Assay Using NanoLuc Luciferase
Authors:  Aleš Berlec, Nika Janež, Meta Sterniša, Anja Klančnik and Jerica Sabotič, date: 02/05/2022, view: 1033, Q&A: 0

Biofilms serve as a bacterial survival strategy, allowing bacteria to persist under adverse environmental conditions. The non-pathogenic Listeria innocua is used as a surrogate organism for the foodborne pathogen Listeria monocytogenes, because they

...
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