Yvon Jaillais
  • Research scientist, CNRS - ENS Lyon
Research fields
  • Plant science
Electro-fusion of Gametes and Subsequent Culture of Zygotes in Rice
Authors:  Erika Toda, Yukinosuke Ohnishi and Takashi Okamoto, date: 12/20/2016, view: 10696, Q&A: 0
Electro-fusion system with isolated gametes has been utilized to dissect fertilization-induced events in angiosperms, such as egg activation, zygote development and early embryogenesis, since the female gametophytes of plants are deeply embedded within ovaries. In this protocol, procedures for isolation of rice gametes, electro-fusion of gametes, and culture of the produced zygotes are described.
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