André Alex Grassmann
  • Post-Doc, University of Connecticut Health Center
Research fields
  • Microbiology
Measurement of FNR-NrdI Interaction by Microscale Thermophoresis (MST)
Authors:  Ingvild Gudim, Marie Lofstad, Marta Hammerstad and Hans-Petter Hersleth, date: 04/20/2017, view: 11100, Q&A: 0
This protocol describes how to measure protein-protein interactions by microscale thermophoresis (MST) using the MonolithTM NT.115 instrument (NanoTemper). We have used the protocol to determine the binding affinities between three different flavodoxin reductases (FNRs) and a flavodoxin-like protein, NrdI, from Bacillus cereus (Lofstad et al., 2016). NrdI is essential in the activation of the manganese-bound form of the class Ib ribonucleotide reductase (RNR) system. RNRs, in turn, are the only source of the de novo synthesis of deoxyribonucleotides required for DNA replication and repair in all living organisms.
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