Junmin He
  • School of Life Sciences, Shaanxi Normal University, China
Research fields
  • Plant science
Gene Dosage Experiments in Enterobacteriaceae Using Arabinose-regulated Promoters
Authors:  Sanchari Bhattacharyya, Shimon Bershtein and Eugene I Shakhnovich, date: 07/20/2017, view: 6965, Q&A: 0
This protocol is used to assay the effect of protein over-expression on fitness of E. coli. It is based on a plasmid expression of a protein of interest from an arabinose-regulated pBAD promoter followed by the measurement of the intracellular protein abundance by Western blot along with the measurement of growth parameters of E. coli cell expressing this protein.
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