Xiao Liu
  • Department of Developmental Biology, Stanford University, USA
Research fields
  • Cell Biology
Collecting and Fixing Nuclear GFP/RFP in L1 Larva for Imaging
Author:  Xiao Liu, date: 03/05/2012, view: 10675, Q&A: 0
In this protocol, L1 stage larvae are collected that carry nuclear-localized GFP/wCherry reporters. These can be fixed so that the GFP/wCherry maintains nuclear localization and stain nuclei by DAPI. This protocol therefore achieves the collection and fixation of nuclei in worm L1 larvae.
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