Isolation of Ribosomal Particles from the Unicellular Cyanobacterium Synechocystis sp. PCC 6803 Authors: Carla V. Galmozzi and
M. Isabel Muro-Pastor ,
date: 03/20/2017,
view: 7947,
Q&A: 0 Isolation of ribosomal particles is an essential step in the study of ribosomal components as well as in the analysis of trans-acting factors that interact with the ribosome to regulate protein synthesis and modulate the expression profile of the cell in response to different environmental conditions. In this protocol, we describe a procedure for the isolation of 70S ribosomes from the unicellular cyanobacterium Synechocystis sp. PCC 6803 (hereafter Synechocystis). We have successfully used this protocol in our study of the cyanobacterial ribosomal-associated protein LrtA, which is a homologue of bacterial HPF (hibernation promoting factor) (Galmozzi et al., 2016).