MM
M. Isabel Muro-Pastor
  • Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC-Universidad de Sevilla, Spain
Research fields
  • Microbiology
Isolation of Ribosomal Particles from the Unicellular Cyanobacterium Synechocystis sp. PCC 6803
Authors:  Carla V. Galmozzi and M. Isabel Muro-Pastor , date: 03/20/2017, view: 7947, Q&A: 0
Isolation of ribosomal particles is an essential step in the study of ribosomal components as well as in the analysis of trans-acting factors that interact with the ribosome to regulate protein synthesis and modulate the expression profile of the cell in response to different environmental conditions. In this protocol, we describe a procedure for the isolation of 70S ribosomes from the unicellular cyanobacterium Synechocystis sp. PCC 6803 (hereafter Synechocystis). We have successfully used this protocol in our study of the cyanobacterial ribosomal-associated protein LrtA, which is a homologue of bacterial HPF (hibernation promoting factor) (Galmozzi et al., 2016).
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