DNA PCR Assays for Igh Rearrangement Authors: Yusuke Satoh,
Takao Sudo and
Takafumi Yokota,
date: 02/20/2014,
view: 9857,
Q&A: 0 This protocol is used for the detection of immunoglobulin heavy (H) chain rearrangements. This PCR-based assay enables detection of DH-JH recombination in cultured hematopoietic cells (Schlissel et al., 1991; Satoh et al., 2013) [e.g. ES-derived cells (Satoh et al., 2013)].