RZ
Roman A Zubarev
Sclerotinia sclerotiorum Protoplast Preparation and Transformation
Authors:  Chi Lan, Lulu Qiao and Dongdong Niu, date: 01/05/2023, view: 907, Q&A: 1

Sclerotinia sclerotiorum causes white mold, leading to substantial losses on a wide variety of hosts around the world. Many genes encoding effector proteins play important roles in the pathogenesis of S. sclerotiorum. Therefore, establishment of a transformation system for the exploration of gene function is necessarily significant. Here, we introduce a modified protocol to acquire protoplasts for transformation and generate knockout strains, which completements the transformation system of S. sclerotiorum.


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