Improve Research Reproducibility
A Bio-protocol resource
Protocols
Biochemistry
Biophysics
Cancer Biology
Cell Biology
Developmental Biology
Immunology
Microbiology
Molecular Biology
Neuroscience
Plant Science
Stem Cell
Systems Biology
Articles and Issues
Current Issue
All Issues
Articles In Press
For Authors
Submission Procedure
Preparation Guidelines
Submit a Protocol
Editorial Process
Editorial Criteria
AI-Generated Material
Publishing Ethics
Competing Interests
Article Processing Charges
About
About Us
Aims & Scope
Advisors
Editors
Reviewers
Leadership and Management
Open Access Policy
Content Availability and Indexing
Journal Partners
Professional Memberships
Contact Us
Alerts
Advanced Search
Submit a Protocol
EN
EN - English
CN - 中文
CN
Log in / Sign up
Bio Page
Edit Profile
Home
Protocols
Biochemistry
Biophysics
Cancer Biology
Cell Biology
Developmental Biology
Immunology
Microbiology
Molecular Biology
Neuroscience
Plant Science
Stem Cell
Systems Biology
Articles and Issues
Current Issue
All Issues
Articles In Press
For Authors
Submission Procedure
Preparation Guidelines
Submit a Protocol
Editorial Process
Editorial Criteria
AI-Generated Material
Publishing Ethics
Competing Interests
Article Processing Charges
About
About Us
Aims & Scope
Advisors
Editors
Reviewers
Leadership and Management
Open Access Policy
Content Availability and Indexing
Journal Partners
Professional Memberships
Contact Us
Alerts
Submit a Protocol
Overview
Authored
(1)
RW
Ryan L. Wallace
Peer-reviewed
Preprint
Ciliary Assembly/Disassembly Assay in Non-transformed Cell Lines
Authors:
Masaki Saito
,
Kensuke Sakaji
,
Wataru Otsu
and
Ching-Hwa Sung
,
date:
03/20/2018,
view:
9313,
Q&A:
0
The primary cilium is a non-motile sensory organelle whose assembly and disassembly are closely associated with cell cycle progression. The primary cilium is elongated from the basal body in quiescent cells and is resorbed as the cells re-enter the cell cycle. Dysregulation of ciliary dynamics has been linked with ciliopathies and other human diseases. The
in vitro
serum-stimulated ciliary assembly/disassembly assay has gained popularity in addressing the functions of the protein-of-interest in ciliary dynamics. Here, we describe a well-tested protocol for transfecting human retinal pigment epithelial cells (RPE-1) and performing ciliary assembly/disassembly assays on the transfected cells.
More >
Find out more
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.