AS
Anil Shaji
  • Faculty, Indian Institute of Science Education and Research, Thiruvananthapuram Thiruvananthapuram
Research fields
  • Biophysics, Biophysics
Transformation of the Cyanobacterium Leptolyngbya boryana by Electroporation
Authors:  Ryoma Tsujimoto, Hiroya Kotani, Aoi Nonaka, Yuri Miyahara, Yuto Hiraide and Yuichi Fujita, date: 12/20/2015, view: 11327, Q&A: 0
Leptolyngbya boryana (L. boryana) (formerly Plectonema boryanum) is a versatile, filamentous cyanobacterium that has the ability to fix nitrogen under microoxic conditions and to grow heterotrophically with glucose in the dark, providing an excellent system to investigate photosynthesis, nitrogen fixation, and their regulatory mechanisms. While L. boryana is not naturally transformable different from the unicellular cyanobacterium Synechocystis sp. PCC 6803, it can be transformed by electroporation. Here we describe the transformation of L. boryana by electroporation to isolate mutants in which a targeted gene is disrupted.
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