Ilenuta Simina Cuciurean
  • MSc Eng in Biotechnology, Biotechnology and Biomedicine Technical University of Denmark
Research fields
  • Plant science
En masse DNA Electroporation for in vivo Transcriptional Assay in Ascidian Embryos
Author:  Sébastien Darras, date: 09/20/2021, view: 2204, Q&A: 0

Ascidian embryos are powerful models for functional genomics, in particular, due to the ease of generating a large number of transgenic embryos by electroporation. In addition, the small size of their genome makes them an attractive model for studying cis-regulatory elements that control gene expression during embryonic development. Here, I describe the adaptation of the seminal method developed 25 years ago in Ciona robusta for en masse DNA electroporation for in vivo transcription to additional species belonging to three genera. It is likely that similar optimizations would make electroporation successful in other ascidian species, where in vitro fertilization can be performed on a large number of eggs.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.