M. Fernanda Palominos
  • Post-Doc, University of California, Berkeley
Research fields
  • Developmental Biology, Evolution, Evodevo
Quantification of Bacteria Residing in Caenorhabditis elegans Intestine
Authors:  M. Fernanda Palominos and Andrea Calixto, date: 05/05/2020, view: 4247, Q&A: 0
Quantification of intestinal colonization by pathogenic or commensal bacteria constitute a critical part of the analysis to understand host-microbe interactions during different time points of their interplay. Here we detail a method to isolate non-pathogenic and pathogenic bacteria from C. elegans intestines, and classify gut phenotypes induced by bacterial pathogens using fluorescently-tagged bacteria. Furthermore, these methods can be used to isolate and identify new culturable bacterial species from natural microbiomes of wild nematodes.
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