YJ
Yunjin Jung
  • College of Pharmacy, Pusan National University, Busan
Isolation and Characterization of Exosomes from Mouse Feces
Authors:  Chunhua Yang, Mingzhen Zhang, Junsik Sung, Lixin Wang, Yunjin Jung and Didier Merlin, date: 04/20/2020, view: 5831, Q&A: 0
Exosomes secreted by colonic epithelial cells are present in feces and contain valuable epigenetic information, such as miRNAs, proteins, and metabolites. An in-depth study of this information is conducive to the diagnosis or treatment of relevant diseases. A crucial prerequisite of such a study is to establish an efficient isolation method, through which we can obtain a relatively more significant amount of exosomes from feces. This protocol is designed to effectively isolate a large number of exosomes from contaminants and other particles in feces by a combined method with fast filtration and sucrose density gradient ultracentrifugation. Exosomes generated by this method are suitable for further RNA, protein, and lipid analysis.
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