Improve Research Reproducibility
A Bio-protocol resource
Protocols
Biochemistry
Biophysics
Cancer Biology
Cell Biology
Developmental Biology
Immunology
Microbiology
Molecular Biology
Neuroscience
Plant Science
Stem Cell
Systems Biology
Articles and Issues
Current Issue
All Issues
Articles In Press
For Authors
Submission Procedure
Preparation Guidelines
Submit a Protocol
Editorial Process
Editorial Criteria
AI-Generated Material
Publishing Ethics
Competing Interests
Article Processing Charges
About
About Us
Aims & Scope
Advisors
Editors
Reviewers
Leadership and Management
Open Access Policy
Content Availability and Indexing
Journal Partners
Professional Memberships
Contact Us
Alerts
Advanced Search
Submit a Protocol
EN
EN - English
CN - 中文
CN
Log in / Sign up
Bio Page
Edit Profile
Home
Protocols
Biochemistry
Biophysics
Cancer Biology
Cell Biology
Developmental Biology
Immunology
Microbiology
Molecular Biology
Neuroscience
Plant Science
Stem Cell
Systems Biology
Articles and Issues
Current Issue
All Issues
Articles In Press
For Authors
Submission Procedure
Preparation Guidelines
Submit a Protocol
Editorial Process
Editorial Criteria
AI-Generated Material
Publishing Ethics
Competing Interests
Article Processing Charges
About
About Us
Aims & Scope
Advisors
Editors
Reviewers
Leadership and Management
Open Access Policy
Content Availability and Indexing
Journal Partners
Professional Memberships
Contact Us
Alerts
Submit a Protocol
Overview
Authored
(1)
SA
Steffen Albrecht
Peer-reviewed
Preprint
ChIP-Seq from Limited Starting Material of K562 Cells and
Drosophila
Neuroblasts Using Tagmentation Assisted Fragmentation Approach
Authors:
Junaid Akhtar
,
Piyush More
and
Steffen Albrecht
,
date:
02/20/2020,
view:
3509,
Q&A:
0
Chromatin immunoprecipitation is extensively used to investigate the epigenetic profile and transcription factor binding sites in the genome. However, when the starting material is limited, the conventional ChIP-Seq approach cannot be implemented. This protocol describes a method that can be used to generate the chromatin profiles from as low as 100 human or 1,000
Drosophila
cells. The method employs tagmentation to fragment the chromatin with concomitant addition of sequencing adaptors. The method generates datasets with high signal to noise ratio and can be subjected to standard tools for ChIP-Seq analysis
More >
Find out more
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.