YO
Yasuo Ohnishi
  • Professor, Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo,2010-
Research fields
  • Microbiology
Preparation of Actinoplanes missouriensis Zoospores and Assay for Their Adherence to Solid Surfaces
Authors:  Takeaki Tezuka, Daisuke Nakane, Tomohiro Kimura and Yasuo Ohnishi, date: 12/20/2019, view: 3275, Q&A: 0
Spherical zoospores of a rare actinomycete, Actinoplanes missouriensis, adhere to various hydrophobic solid surfaces by means of type IV pili. The purpose of this protocol is to provide detailed descriptions of the preparation of A. missouriensis zoospores and an assay for the adhesion of the zoospores to solid surfaces. This adhesion assay, which measures numbers of zoospores that adhered to the dish surface and swimming zoospores in a tunnel chamber by using a phase-contrast microscope, can also be used for swimming cells of other microorganisms.
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