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Overview
Authored
(1)
José Pedro Lopes
Postdoctoral fellow, Clinical Microbiology, Umeå University
Research fields
-
Peer-reviewed
Preprint
Cellulase and Macerozyme-PEG-mediated Transformation of Moss Protoplasts
Authors:
Rituraj Batth
,
Ilenuta Simina Cuciurean
,
Satish Kumar Kodiripaka
,
Sarah Skovlund Rothman
,
Caroline Greisen
and
Henrik Toft Simonsen
,
date:
01/20/2021,
view:
3319,
Q&A:
0
This protocol describes the generation of protoplasts from protonemal tissue of the moss
Physcomitrium patens
(syn.
Physcomitrella patens
), using Cellulase ONOZUKA R10 and Macerozyme R10, followed by polyethylene glycol (PEG) mediated transformation. The protonemal tissue grown in liquid suspension was harvested and treated with enzyme cocktails mix of 1.5% Cellulase ONOZUKA R10 and 0.5% Macerozyme R10 to generate 1,8 million protoplasts within 3 h.
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