Microscopic Observation of Subcellular GFP-tagged Protein Localization in Rice Anthers Authors: Seijiro Ono and
Ken-Ichi Nonomura,
date: 08/05/2018,
view: 4884,
Q&A: 0 This protocol demonstrates a simple method to determine the subcellular localization of fluorescence-tagged proteins on the vibratome sections of rice developing anthers. If a cell type-specific promoter is used to drive the tagged protein-encoding gene, the method enables to clearly distinguish the cells retaining fluorescent signals from other anther cells. It is applicable to both live and fixed samples, and presumably to other plant tissues.