MD
María del Carmen Dacasa Rüdinger
  • Project officer, Division of Forest Genetics and Forest Tree Breeding, Wood and Forestry Competence Centre, Public Enterprise Sachsenforst, Germany, Germany,
Research fields
  • Plant science
Protocol to Test the Effect of Sorbitol in vitro on Hybrid Larch (Larix x eurolepis Henry) Emblings
Authors:  Bettina Johst and María del Carmen Dacasa Rüdinger, date: 07/20/2018, view: 4300, Q&A: 0
This protocol presents a method to test the effect of sorbitol in vitro on hybrid larch plants derived from somatic embryogenesis. We have tested four different media variants, one control variant without sorbitol and three variants of decreasing water potential corresponding to sorbitol concentrations in the culture medium of 4%, 10%, and 20%. We cultured two hybrid larch clones on these media during 35 days and assessed their vitality and weight growth after this time.
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