SK
S.F.Gabriel Krens
  • Institute of Science and Technology (IST) Austria, Austria, Austria,
Research fields
  • Plant science
Protein Expression and Purification of the Hsp90-Cdc37-Cdk4 Kinase Complex from Saccharomyces cerevisiae
Authors:  Kliment A. Verba and David A. Agard, date: 10/05/2017, view: 8266, Q&A: 0
Interactions between Hsp90, its co-chaperone Cdc37 and kinases have been biochemically studied for over three decades and have been shown to be functionally important in organisms from yeast to humans. However, formation of a stable complex for structural studies has been elusive. In this protocol we describe expression and purification of Hsp90-Cdc37-Cdk4 kinase protein complex from Saccharomyces cerevisiae utilizing the viral 2A sequences to titrate the three proteins at similar levels.
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