LD
Luis LP daSilva
  • Department of Cell and Molecular Biology, Ribeirão Preto Medical School, University of São Paulo (FMRP-USP), Brasil, Brasil,
Research fields
  • Biochemistry
Quantification of Colibactin-associated Genotoxicity in HeLa Cells by In Cell Western (ICW) Using γ-H2AX as a Marker
Authors:  Sophie Tronnet and Eric Oswald, date: 03/20/2018, view: 6979, Q&A: 0
The genotoxin colibactin is produced by several species of Enterobacteriaceae. This genotoxin induces DNA damage, cell cycle arrest, senescence and death in eukaryotic cells (Nougayrède et al., 2006; Taieb et al., 2016). Here we describe a method to quantify the genotoxicity of bacteria producing colibactin following a short infection of cultured mammalian cells with colibactin producing E. coli.
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