Isolation of Primary Human Skeletal Muscle Cells Authors: Janelle M. Spinazzola and
Emanuela Gussoni,
date: 11/05/2017,
view: 9241,
Q&A: 0 Primary myoblast culture is a valuable tool in research of muscle disease, pathophysiology, and pharmacology. This protocol describes techniques for dissociation of cells from human skeletal muscle biopsies and enrichment for a highly myogenic population by fluorescence-activated cell sorting (FACS). We also describe methods for assessing myogenicity and population expansion for subsequent in vitro study.