Improve Research Reproducibility
A Bio-protocol resource
Protocols
Biochemistry
Biophysics
Cancer Biology
Cell Biology
Developmental Biology
Immunology
Microbiology
Molecular Biology
Neuroscience
Plant Science
Stem Cell
Systems Biology
Articles and Issues
Current Issue
All Issues
Articles In Press
For Authors
Submission Procedure
Preparation Guidelines
Submit a Protocol
Editorial Process
Editorial Criteria
AI-Generated Material
Publishing Ethics
Competing Interests
Article Processing Charges
About
About Us
Aims & Scope
Advisors
Editors
Reviewers
Leadership and Management
Open Access Policy
Content Availability and Indexing
Journal Partners
Professional Memberships
Contact Us
Alerts
Advanced Search
Submit a Protocol
EN
EN - English
CN - 中文
CN
Log in / Sign up
Bio Page
Edit Profile
Home
Protocols
Biochemistry
Biophysics
Cancer Biology
Cell Biology
Developmental Biology
Immunology
Microbiology
Molecular Biology
Neuroscience
Plant Science
Stem Cell
Systems Biology
Articles and Issues
Current Issue
All Issues
Articles In Press
For Authors
Submission Procedure
Preparation Guidelines
Submit a Protocol
Editorial Process
Editorial Criteria
AI-Generated Material
Publishing Ethics
Competing Interests
Article Processing Charges
About
About Us
Aims & Scope
Advisors
Editors
Reviewers
Leadership and Management
Open Access Policy
Content Availability and Indexing
Journal Partners
Professional Memberships
Contact Us
Alerts
Submit a Protocol
Overview
Authored
(1)
Aurelien A Serandour
Faculty, CR2TI, Ecole Centrale de Nantes, France, France,
Research fields
Molecular Biology
Peer-reviewed
Preprint
Coupling Exonuclease Digestion with Selective Chemical Labeling for Base-resolution Mapping of 5-Hydroxymethylcytosine in Genomic DNA
Authors:
Aurélien A. Sérandour
,
Stéphane Avner
and
Gilles Salbert
,
date:
03/05/2018,
view:
6571,
Q&A:
0
This protocol is designed to obtain base-resolution information on the level of 5-hydroxymethylcytosine (5hmC) in CpGs without the need for bisulfite modification. It relies on (i) the capture of hydroxymethylated sequences by a procedure known as ‘selective chemical labeling’ (see Szulwach
et al.
, 2012) and (ii) the digestion of the captured DNA by exonucleases. After Illumina sequencing of the digested DNA fragments, an
ad hoc
bioinformatic pipeline extracts the information for further downstream analysis.
More >
Find out more
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.