Fish Bile Clean-up for Subsequent Zymography and Mass Spectrometry Proteomic Analyses Author: Rachel Ann Hauser-Davis,
date: 01/20/2018,
view: 6965,
Q&A: 0 Biliary excretion offers a way to analyze various contaminants in aquatic organisms, and fish bile has been used as a biomarker for environmental contamination. The use of the fish bile proteome as a tool for monitoring the impact of environmental contaminants has been recently validated. However, scarce studies in this context are available, and much remains to be investigated. In this context, this protocol describes a fast, reproducible and cheap biliary clean-up procedure for subsequent proteomic analyses, such as zymography and mass spectrometry.