NG
Reviewer
Nicanor González-Morales
  • Research associate, Biology, McGill University, Canada
Research fields
  • Developmental biology
Bimolecular Fluorescence Complementation (BiFC) for Studying Sarcomeric Protein Interactions in Drosophila
Authors:  Océane Marescal, Frieder Schöck and Nicanor González-Morales, date: 04/05/2020, view: 4847, Q&A: 0
Protein-protein interactions in Drosophila myofibrils are essential for their function and formation. Bimolecular Fluorescence Complementation (BiFC) is an effective method for studying protein interactions and localization. BiFC relies on the reconstitution of a monomeric fluorescent protein from two half-fragments when in proximity. Two proteins tagged with the different half-fragments emit a fluorescent signal when they are in physical contact, thus revealing a protein interaction and its spatial distribution. Because myofibrils are large networks of interconnected proteins, BIFC is an ideal method to study protein-protein interactions in myofibrils. Here we present a protocol for generating transgenic flies compatible with BiFC and a method for analyzing protein-protein interactions based on the fluorescent BiFC signal in myofibrils. Our protocol is applicable to the majority of Drosophila proteins and with few modifications may be used to study any tissue.
Rapid IFM Dissection for Visualizing Fluorescently Tagged Sarcomeric Proteins
Authors:  Yu Shu Xiao, Frieder Schöck and Nicanor González-Morales, date: 11/20/2017, view: 9765, Q&A: 0
Sarcomeres, the smallest contractile unit of muscles, are arguably the most impressive actomyosin structure. Yet a complete understanding of sarcomere formation and maintenance is missing. The Drosophila indirect flight muscle (IFM) has proven to be a very valuable model to study sarcomeres. Here, we present a protocol for the rapid dissection of IFM and analysis of sarcomeres using fluorescently tagged proteins.
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