Preparation of Crude Synaptosomal Fractions from Mouse Brains and Spinal Cords Author: Oliver Wirths,
date: 08/05/2017,
view: 13841,
Q&A: 0 The current protocol describes the preparation of crude synaptosomal fractions from mouse brain or spinal cord samples. In detail, a sequential protocol yielding crude synaptosomal and light membrane fractions is provided. This fast and easy method might be sufficient to assess the amount of synaptic proteins in down-steam applications like Western-blot or ELISA in e.g., mouse models of Alzheimer’s disease or other neurodegenerative conditions.