Oliver Wirths
  • Molecular Psychiatry, Dept. of Psychiatry and Psychotherapy, University Medical Center Göttingen (UMG), Germany
Research fields
  • Neuroscience
Preparation of Crude Synaptosomal Fractions from Mouse Brains and Spinal Cords
Author:  Oliver Wirths, date: 08/05/2017, view: 13841, Q&A: 0
The current protocol describes the preparation of crude synaptosomal fractions from mouse brain or spinal cord samples. In detail, a sequential protocol yielding crude synaptosomal and light membrane fractions is provided. This fast and easy method might be sufficient to assess the amount of synaptic proteins in down-steam applications like Western-blot or ELISA in e.g., mouse models of Alzheimer’s disease or other neurodegenerative conditions.
Extraction of Soluble and Insoluble Protein Fractions from Mouse Brains and Spinal Cords
Author:  Oliver Wirths, date: 08/05/2017, view: 27276, Q&A: 3
The current protocol details the preparation of soluble and insoluble protein lysates from mouse brain or spinal cord samples. In detail, tissue homogenization and sequential protein extraction are described. This procedure yields soluble and insoluble protein extracts that can be further processed in down-stream applications like ELISA or Western blotting.
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