Xiaojun Ren
  • Department of Chemistry, University of Colorado Denver, USA
Research fields
  • Microbiology
Labelling HaloTag Fusion Proteins with HaloTag Ligand in Living Cells
Authors:  Huy Nguyen Duc and Xiaojun Ren , date: 09/05/2017, view: 10927, Q&A: 0
HaloTag has been widely used to label proteins in vitro and in vivo (Los et al., 2008). In this protocol, we describe labelling HaloTag-Cbx fusion proteins by HaloTag ligands for live-cell single-molecule imaging (Zhen et al., 2016).
Producing GST-Cbx7 Fusion Proteins from Escherichia coli
Authors:  Thao Ngoc Huynh and Xiaojun Ren , date: 06/20/2017, view: 10199, Q&A: 1
This protocol describes the production of GST-Cbx7 fusion proteins from E. coli, originally developed in the recent publication (Zhen et al., 2016). The pGEX-6P-1-GST plasmids encoding the Cbx7 variants were transformed into BL21 competent cells. The fusion protein production was induced by isopropyl-beta-D-thiogalactopyranoside and they were purified by Glutathione Sepharose 4B. This protocol can be adapted for the purification of other proteins.
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