JG
Jiasong Guo
  • Department of Histology and Embryology, Southern Medical University, Guangdong, Guangzhou
Isolation and Purification of Schwann Cells from Spinal Nerves of Neonatal Rat
Authors:  Jinkun Wen, Dandan Tan, Lixia Li and Jiasong Guo, date: 10/20/2017, view: 11736, Q&A: 0
Primary cultured Schwann cells (SCs) are widely used in the investigation of the biology of SC and are important seed cells for neural tissue engineering. Here, we describe a novel protocol for harvesting primary cultured SCs from neonatal Sprague-Dawley (SD) rats. In the present protocol, dissociated SCs are isolated from the spinal nerves of neonatal rats and purified by the treatment of cytosine arabinoside (AraC).
Preparation of Teased Nerve Fibers from Rat Sciatic Nerve
Authors:  Jinkun Wen, Lixia Li, Dandan Tan and Jiasong Guo, date: 10/05/2017, view: 11094, Q&A: 0
Compared to tissue sectioning techniques, the technique of teasing single nerve fibers provides a better way to understand the structures of myelin sheaths and axons of the peripheral myelinated nerves. This protocol describes a method for preparation of teased single nerve fibers from rat sciatic nerve. In this protocol, fixed nerves are teased into single individual fibers and arranged onto adhesion microscope slides for further immuno-staining.
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