Ning Yang
  • Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, China
Research fields
  • Plant science
Protein Isolation from Plasma Membrane, Digestion and Processing for Strong Cation Exchange Fractionation
Authors:  Ning Yang, Bing Han and Tai Wang, date: 05/20/2017, view: 10327, Q&A: 0
Plasma membrane (PM) proteins play crucial roles in diverse biological processes. But their low abundance, alkalinity and hydrophobicity make their isolation a difficult task. This protocol describes an efficient method for PM proteins isolation, digestion and fractionation so that they can be well prepared for mass spectrometry analysis.
Plasma Membrane Preparation from Lilium davidii and Oryza sativa Mature and Germinated Pollen
Authors:  Ning Yang, Bing Han, Lingtong Liu, Hao Yang and Tai Wang, date: 05/20/2017, view: 7929, Q&A: 0
Pollen germination is an excellent process to study cell polarity establishment. During this process, the tip-growing pollen tube will start elongating. The plasma membrane as the selectively permeable barrier that separates the inner and outer cell environment plays crucial roles in this process. This protocol described an efficient aqueous polymer two-phase system followed by alkaline solution washing to prepare Lilium davidii or Oryza sativa plasma membrane with high purity.
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