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Protocols
Biochemistry
Biophysics
Cancer Biology
Cell Biology
Developmental Biology
Immunology
Microbiology
Molecular Biology
Neuroscience
Plant Science
Stem Cell
Systems Biology
Articles and Issues
Current Issue
All Issues
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For Authors
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Preparation Guidelines
Submit a Protocol
Editorial Process
Editorial Criteria
AI-Generated Material
Publishing Ethics
Competing Interests
Article Processing Charges
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About Us
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Submit a Protocol
Overview
Authored
(2)
Ning Yang
Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, China
Research fields
Plant science
Peer-reviewed
Preprint
Protein Isolation from Plasma Membrane, Digestion and Processing for Strong Cation Exchange Fractionation
Authors:
Ning Yang
,
Bing Han
and
Tai Wang
,
date:
05/20/2017,
view:
10327,
Q&A:
0
Plasma membrane (PM) proteins play crucial roles in diverse biological processes. But their low abundance, alkalinity and hydrophobicity make their isolation a difficult task. This protocol describes an efficient method for PM proteins isolation, digestion and fractionation so that they can be well prepared for mass spectrometry analysis.
More >
Plasma Membrane Preparation from
Lilium davidii
and
Oryza sativa
Mature and Germinated Pollen
Authors:
Ning Yang
,
Bing Han
,
Lingtong Liu
,
Hao Yang
and
Tai Wang
,
date:
05/20/2017,
view:
7929,
Q&A:
0
Pollen germination is an excellent process to study cell polarity establishment. During this process, the tip-growing pollen tube will start elongating. The plasma membrane as the selectively permeable barrier that separates the inner and outer cell environment plays crucial roles in this process. This protocol described an efficient aqueous polymer two-phase system followed by alkaline solution washing to prepare
Lilium davidii
or
Oryza sativa
plasma membrane with high purity.
More >
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