Jérôme Lugrin
  • Department of Biochemistry, University of Lausanne, Switzerland
Research fields
  • Immunology
Detection of ASC Oligomerization by Western Blotting
Authors:  Jérôme Lugrin and Fabio Martinon , date: 05/20/2017, view: 21165, Q&A: 0
The apoptosis-associated speck-like protein with a caspase-recruitment domain (ASC) adaptor protein bridges inflammasome sensors and caspase-1. Upon inflammasome activation, ASC nucleates in a prion-like manner into a large and single platform responsible for the recruitment and the activation of caspase-1. Active caspase-1 will in turn promote the proteolytic maturation of the pro-inflammatory cytokine IL-1β. ASC oligomerization is direct evidence for inflammasome activation and its detection allows a read-out independent of caspase-1 and IL-1β. This protocol describes how to detect the oligomerization of ASC by Western blot.
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