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Overview
Authored
(1)
Sophie Tronnet
The Laboratory for Molecular Infection Medicine Sweden (MIMS)
Research fields
Microbiology
Peer-reviewed
Preprint
Quantification of Colibactin-associated Genotoxicity in HeLa Cells by In Cell Western (ICW) Using
γ
-H2AX as a Marker
Authors:
Sophie Tronnet
and
Eric Oswald
,
date:
03/20/2018,
view:
6979,
Q&A:
0
The genotoxin colibactin is produced by several species of
Enterobacteriaceae
. This genotoxin induces DNA damage, cell cycle arrest, senescence and death in eukaryotic cells (Nougayrède
et al.
, 2006; Taieb
et al.
, 2016). Here we describe a method to quantify the genotoxicity of bacteria producing colibactin following a short infection of cultured mammalian cells with colibactin producing
E. coli
.
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