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Overview
Authored
(1)
Reviewed
(13)
Emmanuel Orta-Zavalza
Faculty, Universidad Autónoma de Ciudad Juárez
Research fields
Cell Biology, Immunology, Microbiology, Molecular Biology, Mycology
Peer-reviewed
Preprint
Catalase Activity Assay in
Candida glabrata
Authors:
Emmanuel Orta-Zavalza
,
Marcela Briones-Martin-del-Campo
,
Irene Castano
and
Alejandro De Las Penas
,
date:
03/20/2014,
view:
18747,
Q&A:
1
Commensal and pathogenic fungi are exposed to hydrogen peroxide (H
2
O
2
) produced by macrophages of the host. Pathogenic fungi counteract the harmful effects of H
2
O
2
with the enzyme catalase (EC 1.11.1.6), which decomposes two molecules of H
2
O
2
to two molecules of H
2
O and O
2
. Contribution of antioxidant systems on fungal virulence is actively studied. Measurement of catalase activity can contribute to the elucidation of the factors that influence the regulation of this pivotal enzyme. Here we describe a simple spectrophotometric method in which the activity of catalase is measured in total yeast extracts. Decomposition of H
2
O
2
by the yeast extract is followed by the decrease in absorbance at 240 nm. The difference in absorbance through time (ΔA
240
) is inferred as the measure of catalase activity.
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