André Vidal-Meireles
  • Institute of Plant Biology, Biological Research Centre of the Hungarian Academy of Sciences, Hungary
Research fields
  • Plant science
Isolation of Murine Alveolar Type II Epithelial Cells
Authors:  Fan Sun, Gutian Xiao and Zhaoxia Qu, date: 05/20/2017, view: 11290, Q&A: 0
We have optimized a protocol for isolation of alveolar type II epithelial cells from mouse lung. Lung cell suspensions are prepared by intratracheal instillation of dispase and agarose followed by mechanical disaggregation of the lungs. Alveolar type II epithelial cells are purified from these lung cell suspensions through magnetic-based negative selection using a Biotin-antibody, Streptavidin-MicroBeads system. The purified alveolar type II epithelial cells can be cultured and maintained on fibronectin-coated plates in DMEM with 10% FBS. This protocol enables specific investigation of alveolar type II epithelial cells at molecular and cellular levels and provides an important tool to investigate in vitro the mechanisms underlying lung pathogenesis.
Murine Bronchoalveolar Lavage
Authors:  Fan Sun, Gutian Xiao and Zhaoxia Qu, date: 05/20/2017, view: 25361, Q&A: 1
A basic Bronchoalveolar lavage (BAL) procedure in mouse is described here. Cells and fluids obtained from BAL can be analyzed by Hema3-staining, immunostaining, Fluorescence-activated cell sorting (FACS), PCR, bicinchoninic acid protein assay, enzyme-linked immunosorbent assay (ELISA), luminex assays, etc., to examine the immune cells, pathogens, proteins such as cytokines/chemokines, and the expression levels of inflammation-related and other genes in the cells. This will help to understand the underlying mechanisms of these lung diseases and develop specific and effective drugs.
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