Guo Chen
  • Division of Cancer Biology, Department of Radiation Oncology, Emory University School of Medicine and Winship Cancer Institute of Emory University, USA
Research fields
  • Cancer biology
Purification of Total RNA from DSS-treated Murine Tissue via Lithium Chloride Precipitation
Authors:  Emilie Viennois, Anika Tahsin and Didier Merlin, date: 05/05/2018, view: 8119, Q&A: 0
We have developed a protocol to purify RNA from DSS (Dextran Sulfate Sodium)-treated mouse tissues. This method, which prevents downstream inhibition of q-RT-PCR observed in DSS-treated tissues, relies on successive precipitations with lithium chloride.
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