MK
Martina Kolb
  • Max Planck Institute for Developmental Biology, Department of Cell Biology, Germany
Research fields
  • Plant science
Use of SCRI Renaissance 2200 (SR2200) as a Versatile Dye for Imaging of Developing Embryos, Whole Ovules, Pollen Tubes and Roots
Authors:  Thomas J. Musielak, Patrick Bürgel, Martina Kolb and Martin Bayer, date: 09/20/2016, view: 17981, Q&A: 1
Confocal laser scanning microscopy in combination with fluorescent proteins is a powerful tool for the study of sexual reproduction and other developmental processes in plants. In order to understand the origin and localization of fluorescent signals in a complex tissue, staining of cell outlines is often mandatory. Cell wall staining with SCRI Renaissance 2200 (SR2200) has recently been described as a method of choice to study plant reproductive processes (Musielak et al., 2015). In this protocol, we present detailed instructions on the use of SR2200 to stain cell walls in different Arabidopsis tissues.
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