Melina Amenta
  • Área Biomolecular, Unidad Integrada Balcarce, Instituto Nacional de Tecnología Agropecuaria-Universidad Nacional de Mar del Plata, Argentina
Research fields
  • Plant science
A Protocol to Measure the Cytoplasmic Calcium in Arabidopsis Guard Cells
Authors:  Li Li, Feng Lin, Yana Qu and Qun Zhang, date: 05/05/2015, view: 10548, Q&A: 1
Cytoplasmic calcium ([Ca2+]cyt) acts as a stimulus-induced second messenger in multiple signal transduction cascades (Allen et al., 1999). In plant cells, a dramatic and readily assayed response to stimulus is the change of stomatal aperture. Changes in [Ca2+]cyt of stomatal guard cells were involved in stomatal movement in response to various stimuli and cellular processes. In general, there are two available ways to measure [Ca2+]cyt in guard cells, i.e., loading of calcium-sensitive fluorescence dyes such as fluo-3 AM and fura-2 or expressing genetically encoded calcium indicators such as yellow cameleon (Krebs et al., 2012). In this protocol, we aim at describing the experimental procedure to record [Ca2+]cyt fluctuation in guard cells with loading of fluo-3 AM upon ABA or PA treatment combining with fluorescence imaging performed with confocal laser scanning microscope.
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.