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Overview
Authored
(1)
PF
Primrose P. E. Freestone
Department of Infection, Immunity and Inflammation, University of Leicester, UK
Research fields
Microbiology
Peer-reviewed
Preprint
Protein Expression and Purification of the Hsp90-Cdc37-Cdk4 Kinase Complex from
Saccharomyces cerevisiae
Authors:
Kliment A. Verba
and
David A. Agard
,
date:
10/05/2017,
view:
8266,
Q&A:
0
Interactions between Hsp90, its co-chaperone Cdc37 and kinases have been biochemically studied for over three decades and have been shown to be functionally important in organisms from yeast to humans. However, formation of a stable complex for structural studies has been elusive. In this protocol we describe expression and purification of Hsp90-Cdc37-Cdk4 kinase protein complex from
Saccharomyces cerevisiae
utilizing the viral 2A sequences to titrate the three proteins at similar levels.
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