Hong Bae Jeon
  • Biomedical Research Institute, R&D Center, MEDIPOST Co., Ltd., Republic of Korea
Research fields
  • Stem cell
In vitro Chondrogenic Hypertrophy Induction of Mesenchymal Stem Cells
Authors:  Sang Young Jeong, Miyoung Lee, Soo Jin Choi, Wonil Oh and Hong Bae Jeon, date: 12/05/2016, view: 12298, Q&A: 1
To investigate underlying mechanism of chondrogenic hypertrophy, we need proper in vitro hypertrophic model of mesenchymal stem cells (MSCs). This protocol describes our defined method for induction of in vitro chondrogenic hypertrophy of human umbilical cord blood-derived MSCs (hUCB-MSCs). By adding thyroid hormone (T3; triiodothyronine) and minimum osteogenic-inducing factors to culture medium, we could induce hypertrophy of hUCB-MSCs in vitro. Hypertrophic induction was validated using immunohistochemical analysis, Western blotting and reverse transcriptase polymerase chain reaction.
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