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Biochemistry
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Overview
Authored
(1)
SK
Shao-Qing Kuang
Department of Internal Medicine, Division of Medical Genetics, University of Texas Health Science Center, USA
Research fields
Developmental biology
Peer-reviewed
Preprint
Vascular Smooth Muscle Cell Isolation and Culture from Mouse Aorta
Authors:
Callie S. Kwartler
,
Ping Zhou
,
Shao-Qing Kuang
,
Xue-Yan Duan
,
Limin Gong
and
Dianna M. Milewicz
,
date:
12/05/2016,
view:
21331,
Q&A:
0
Vascular smooth muscle cells (SMC) in the ascending thoracic aorta arise from neural crest cells, whereas SMCs in the descending aorta are derived from the presomitic mesoderm. SMCs play important roles in cardiovascular development and aortic aneurysm formation. This protocol describes the detailed process for explanting ascending and descending SMCs from mouse aortic tissue. Conditions for maintenance and subculture of isolated SMCs and characterization of the vascular SMC phenotype are also described.
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